Genome Editing, Transgenic, and Virus Core (GETV)
The Genome Editing, Transgenic and Virus core (GETV) is dedicated to shepherding projects, from vector design to the establishment and maintenance of valuable transgenic/knockout/knockin lines. The core assists investigators by developing state-of-the-art strategies to solve complex biological problems using transgenic, gene editing and viral vector approaches. Directed by Dr. Kyle Orwig and managed by Dr. Yi Sheng, the core provides technical expertise, advice and services for investigators in the University of Pittsburgh community and beyond. Please see the website or contact us for further information or to schedule a face to face meeting to design and implement your experimental plans.
- Assistance for generating recombinant plasmids;
- Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work;
- Mouse and rat transgenesis, including transgenic and knockout/knockin techniques using pronuclear injection, ES cell injection/chimera and CRISPR/Cas9-mediated genome editing;
- Lentivirus, adenovirus and adeno-associated virus vectors;
- Cryopreserve or re-derive of valuable transgenic/knockout or other rodent lines using sperm or embryos;
- Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines;
- GETV core services are approved by the Institutional Animal Care and Use Committee (IACUC), the recombinant DNA committee, and the environmental health and safety office of the University of Pittsburgh.