Directory of Laboratory and Computing Research Resources
Division of Laboratory Animal Resources (DLAR)
The Division of Laboratory Animal Resources (DLAR) provides education, training, veterinary and animal husbandry care, and administrative services to support the institution’s animal based research and teaching needs. The DLAR coordinates efforts to provide a humane, quality animal care program in compliance with legal and regulatory requirements. The programs and facilities are USDA registered, covered under an Assurance with the Office of Lab Animal Welfare (OLAW) of the PHS, and accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC). The DLAR forms and guidance documents may be found. Additional animal care policies may be found on the Institutional Animal Care and Use Committee’s website.
Biomedical Research Imaging Resources Directory
This website provides searchable directory of the imaging resources and capabilities available to biomedical researchers at the University of Pittsburgh and Carnegie Mellon University.
- Biological Sciences Microscopy and Imaging Facility
- Center for Biologic Imaging (Oakland site)
- Center for Ultrasound Molecular Imaging and Therapeutics
- Department of Neurobiology Microscopes
- Department of Structural Biology Cryo-electron Microscopy (CryoEM) Facility
- Division of Laboratory Animals Research (DLAR) Imaging
- Drug Discovery Institute (UPDDI) - High Content Screening (HCS) Imaging Resources
- Laboratory of Physical Molecular Biology (chromatin fiber imaging)
- Magnetic Resonance Research Center (MRRC)
- Near Infrared Spectroscopy (NIRS) Brain Imaging Laboratory
- Neuroscience Imaging Center (NIC)/Neuroimaging Laboratory, McGowan Institute of Regenerative Medicine
- Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute Small Animal Ultrasonography Core
- Positron Emission Tomography (PET) Facility
- Rangos Research Center Animal Imaging Core Facility, Children’s Hospital of Pittsburgh of UPMC
- Rangos Research Center Cell Imaging Core I and II Laboratories, Children’s Hospital of Pittsburgh of UPMC
- Structural Biology Nuclear Magnetic Resonance (NMR) Facility
- UPMC Hillman Cancer Center Cell and Tissue Imaging Facility (CTIF)
- UPMC Hillman Cancer Center In Vivo Imaging Facility (IVIF) - Preclinical PET and PET-CT
- UPMC Hillman Cancer Center In Vivo Imaging Facility (IVIF) – Preclinical MRI
- UPMC Hillman Cancer Center In Vivo Imaging Facility (IVIF) – Preclinical Optical Imaging
- UPMC Hillman Cancer Center In Vivo Imaging Facility (IVIF) – Preclinical Ultrasound Imaging
Small Molecule Biomarker Core (SMBC)
The SMBC utilizes state-of-the-art instrumentation to provide quantitative analysis of specific analytes in clinical samples and method development for analytes where a method does not currently exist. The highly sensitive and specific techniques employed by the Core provide accurate quantitation for multiple biomarkers from the same sample, which maximizes the ability to link basic science with clinical outcomes.
Methods are currently available to analyze many compounds including: cyclooxygenase, CYP450 and lipoxygenase metabolites of arachidonic acid, progestins, estrogens, neurosteroids, and vitamin D metabolites. A more detailed overview, including current methods available from the core, is available at the web site.
Peptide and Peptoid Synthesis Core
The Peptide and Peptoid Synthesis Core provides comprehensive services for synthesis, purification, and characterization of synthetic peptides and peptoids. State-of-the-art microwave technology enables the high quality production of long and otherwise difficult to synthesize sequences. The purity and integrity of each synthesis product is confirmed by HPLC and mass spectrometry.
Facility personnel are available for consultation with investigators regarding the design of synthesis peptides and peptoids, estimates of yield, and considerations of purity requirements. Peptides and peptoids can be produced at standard scales of 0.025 mmol (10-20mg), 0.05 mmol (20 -40 mg), 0.1 mmol (50-100mg), 0.2 mmol (100-200mg), and 0.5 mmol (300-500mg), with actual yields dependent on peptide length and content.
Peptides can be prepared with custom modifications including but not limited to acetylation, biotinylation, phosphorylation, cyclization, and fluorescent dyes. The facility has the capacity to produce certified peptides for use in phase I and II human clinical trials with appropriate production documentation for submission to the FDA and other regulatory agencies.
Pitt Biospecimen Core (PBC)
The PBC provides University of Pittsburgh researchers with excess tissue materials obtained from clinical procedures performed at some of the University of Pittsburgh Medical Center (UPMC) hospitals. The main purposes of the PBC are: (1) to provide a mechanism to simplify and streamline the process of research tissue accrual, storage and disbursement and (2) to provide efficient research pathology support services.
Services provided by PBC include:
- Human tissue and biological specimen procurement services
- Honest broker services
- Research histology services
- Annotation of clinical data
- Tissue microarray (TMA) services
The PBC contains a variety of solid tissues, cell aspirates, blood, peripheral blood mononuclear cells, as well as other clinical samples. Solid tissues are available from an assortment of tumors and from various non-neoplastic disease states from sources including the gastrointestinal tract, liver, and lung.
True normal tissues procured for approved projects through a working relationship with the Center for Organ Recovery and Education (CORE) are also available. The PBC develops procurement strategies on an ongoing basis for projects requiring tissue types or disease states that are not currently being collected. This effort includes working directly with investigators as an interface with clinics, surgical programs and the PBC Rapid Autopsy Program.
The PBC has IRB approval for a variety of collections including a specific PBC IRB to collect tissues that would have otherwise been discarded. The PBC provides service based on the existing PBC IRB protocol if appropriate; it can assist investigators with submission of their own IRB protocols, when necessary.
(Formerly Health Sciences Tissue Bank - HSTB)
Genomics Research Core
The Genomics Research Core is equipped with state-of-the-art instrumentation and provides a variety of standard as well as customized genomic analyses to University researchers. The services include Sanger DNA sequencing, Next Generation sequencing of DNA and RNA, RNA/DNA extraction, purification and QC services, gene expression microarrays, whole transcriptome human and mouse microarrays, miRNA microarrays, TaqMan real-time PCR and Nanostring digital expression profiling for mRNA and miRNA, candidate gene and whole genome SNP array genotyping and NanoString CNV analysis. New technology and capabilities are added frequently; for a complete description of services and pricing, please visit the website.
The laboratory staff and leadership are available to provide expert advice and support with experimental design, new protocol development, technical support, data analysis and interpretation and assistance with manuscript and grant preparation including budgeting related to Genomics Research Core Services.
Biomedical Mass Spectrometry Center
The Biomedical Mass Spectrometry (BioMS) Center is a campus-wide shared facility dedicated to advancing the use and application of mass spectrometry in basic, translational, and clinical research. The center combines state-of-the-art high resolution mass spectrometry with classical biochemical approaches to identify and quantify biologically relevant proteins.
Not sure how to get started?
The BioMS center helps newcomers get started fast, by offering an in-gel protein identification service. Cut out your coomassie stained gel band and get it to us by noon on Monday and we will send you a list of identified proteins and post-translational modifications the following week.
Make us an extension of your lab.
Sometimes there is no substitute for doing it yourself. Faculty and students that want to gain first hand mass spectrometry experience are encouraged to work alongside the expert staff and other users in the center. We provide the bench space, instrumentation, and expertise. You provide the excitement and energy.
Want to discuss a specific project?
Nathan Yates (Center Director) and Xuemei Zeng (Asst. Director for Proteomics) regularly meet with investigators to discuss and plan collaborative research projects. To schedule a meeting, contact Dr. Nathan Yates.