Directory of Laboratory and Computing Research Resources
Drug Discovery Institute (UPDDI)
Vision for the University of PIttsburgh Drug Discovery Institute: Optimizing the discovery and development of new molecular entities through collaborations between faculty, academic centers, corporations and government agencies, together with innovations focused on Quantitative Systems Pharmacology (QSP).
The UPDDI has three major classes of activities: a) integrated programs that apply QSP to metastatic breast cancer, Huntington’s Disease (as a first focus area to be extended to other neurodegenerative diseases) and liver diseases; b) projects based on ligand- or target-centric discovery that are driven by faculty collaborators/ RO1 grants covering many therapeutic areas; and c) translational technology development including companion diagnostics, computational biology/chemistry tools, analytics to identify and quantify heterogeneity in experimental and clinical samples and advanced human tissue models for discovery. The UPDDI is positioned in an outstanding environment to translate key scientific and clinical advances into novel therapeutics.
Transgenics and Gene Targeting
The TGT (Transgenic and Gene Targeting) and Innovative Technologies Development (ITD) Cores in the Department of Immunology are a shared resource designed to produce genetically engineered mice for investigators of University of Pittsburgh. Our laboratory provides a broad, comprehensive range of services to investigators.
Keywords: Animal, genetic, vector, CRISPR/Cas9, rederivation, mouse, cryopreserve, knockin/knockout, chimera, chimeric
Virus Production (Recombinant)
Viral Vector Production
The Genome Editing, Transgenic, and Virus Core (GETV) facility provides lentivirus, adenovirus and adeno-associated virus vector design assistance and production. The Core maintains an inventory of stock plasmids and viral vectors that are available for a nominal fee.
Lentiviral vectors are produced in 293FT cells using a four plasmid system and are replication defective. Lentiviral vectors prepared using this system can be used at BSL2.
Adeno-associated virus 2 (AAV2) is produced in 293 cells and all vectors are purified for distribution. Purity is confirmed by SDS-PAGE and densitometry. A certificate of analysis is available upon request. AAV vectors prepared in this way are suitable for work at biosafety level 1 (BSL1).
Adenoviral vectors are produced in 293AD cells and purified for distribution. Adenoviral vectors prepared using this system can be used at BSL2.
For more information and to place an order link to the order form.
Health Sciences Core Research Facilities
The Health Sciences Core Research Facilities (HSCRF) were established through the Office of the Senior Vice Chancellor, Health Sciences in recognition of the vital role that centralized research resources play in the academic research enterprise. The technology required to perform innovative biomedical research changes rapidly and centralized research resources provide access to expertise, technology applications, and state-of-the-art instruments that are otherwise unavailable to most investigators.
Our research resource facilities include scientists and technicians who provide expert advice for study design, standard and custom applications and assist the investigator to take their project aims from conception to data production. The scientific leadership is available to work with you to adapt and apply methods appropriate to your scientific goals and assure that the interpretation of the results is supported by the data.
- Biomedical Mass Spectrometry Center (Proteomics, quantitation & other)
- Genomics Analysis Core (Bioinformatics)
- Genomics Research Core (RNA, DNA, Nextgen Sequencing NGS)
- Health Sciences Metabolomics and Lipidomics Core
- Health Sciences Sequencing Core at Children's Hospital of Pittsburgh (Illumina sequencing NGS)
- Pitt Biospecimen Core (formerly Health Sciences Tissue Bank)
- Peptide and Peptoid Synthesis Core
- Small Molecule Biomarker Core (Quantitative Mass Spectrometry analysis)
Dietrich School Scientific Stockroom
The Dietriech School Scientific Stockroom (DSS) is an award winning combined stockroom that opened May 20, 2015. The catalog includes products from the former Biological Sciences, Chemistry, and Physics stockrooms, as well as the Excise Tax Free Ethanol Program all in one convenient location. It is a University owned and run internal scientific supplier with inventory management support from Unity Lab Services. New suppliers and products are added regularly. You can pick up your order in the Chevron building or Langley Hall or have them deliver to your building next day. To Order from the DSS Use the DSS Punch-out on PantherExpress via my.pitt.edu.
326 Chevron Science Center
7:30 a.m. to 5 p.m. Monday - Friday
412-624-8551 or 412-624-4260
LANGLEY STATON (alternative stockroom pickup location)
120F Langley Hall/Garage
8 a.m. to 4:30 p.m. Monday - Friday
Genome Editing, Transgenic, and Virus Core (GETV)
The Genome Editing, Transgenic and Virus core (GETV) is dedicated to shepherding projects, from vector design to the establishment and maintenance of valuable transgenic/knockout/knockin lines. The core assists investigators by developing state-of-the-art strategies to solve complex biological problems using transgenic, gene editing and viral vector approaches. Directed by Dr. Kyle Orwig and managed by Dr. Yi Sheng, the core provides technical expertise, advice and services for investigators in the University of Pittsburgh community and beyond. Please see the website or contact us for further information or to schedule a face to face meeting to design and implement your experimental plans.
- Assistance for generating recombinant plasmids;
- Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work;
- Mouse and rat transgenesis, including transgenic and knockout/knockin techniques using pronuclear injection, ES cell injection/chimera and CRISPR/Cas9-mediated genome editing;
- Lentivirus, adenovirus and adeno-associated virus vectors;
- Cryopreserve or re-derive of valuable transgenic/knockout or other rodent lines using sperm or embryos;
- Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines;
- GETV core services are approved by the Institutional Animal Care and Use Committee (IACUC), the recombinant DNA committee, and the environmental health and safety office of the University of Pittsburgh.
Unified Flow Core
The Unified Flow Core provides state-of-the-art flow cytometry and cell sorting services to the entire University of Pittsburgh research community as well as researchers at neighboring institutions. A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves.
The Unified Flow Core has 3 LSR Fortessas (1 bio-contained), 2 LSR IIs, 3 FACSAria cell sorters (2 bio-contained) and 1 ImageStreamx Mark II
Magee Womens Research Institute (MWRI) Core Services
For a complete up to date lising and more information about each of the MWRI Cores please go to the research services web link.
- Obstetric Medical and Infant (MOMI) Database and Biobank
- Histology & Micro-Imaging Core (HMC)
- Genome Editing, Transgenic & Virus (GETV) Core
- Cell Cytometry and Sorting Core
- Confocal Microscope Core
- Clinical and Translational
Rangos (Children’s Hospital of Pittsburgh) Core Facilities
For a complete listing of research resources available through the Children’s Hospital of Pittsburgh (CHP), please link to their Core Facilities web page.
- Cell Imaging Core Laboratory - Equipped with confocal microscopes and devices for live cell imaging and laser capture microdissection.
- Flow Cytometry Core Laboratory - Analytical flow cytometry and sterile cell sorting equipment and services.
- Histology Core Laboratory - Support to investigators in need of histology-based technical services and pathological interpretation.
- Metabolic Core Laboratory - Analytical services to support research in energy metabolism, metabolic pathways, and cellular metabolic function including mass spectrometry (LC/MS/MS), Seahorse® analyzer, amino acid analyzer, and reverse-phase HPLC system.
- Animal Imaging Core Laboratory - Including “micro” magnetic resonance imaging (MRI) and Computer Tomography (CT) systems, high-resolution ultrasound and bioluminescence systems.
- Bioinformatics Core Facility - Study design implementation support, including high-throughput data analysis, statistical support, proposal writing and grantsmanship as well as faculty collaborations.
Health Sciences Sequencing Core at Children's Hospital of Pittsburgh
The Health Sciences Sequencing Core at Children’s Hospital of Pittsburgh (HSSCatCHP) offers Illumina next generation sequencing services to the University of Pittsburgh research community. The core was established by a cooperative agreement between the University of Pittsburgh Schools of Health Sciences and the Children’s Hospital of Pittsburgh of UPMC.
Our personnel are experts in next generation sequencing technologies and are committed to remaining abreast of advances that will impact the research effort of investigators. We encourage you to contact our core managers Debby Hollingshead and Will MacDonald to arrange for a consultation. Our staff is available to share their experience and expertise to assist with development of projects of all sizes, standard or custom assay design, and other specialized application needs.