Research Resources - Laboratory and Computing

This directory provides links or contact information for laboratory, computing, and data analysis resources that are available to University investigators. 

To submit updates, or to request the addition of a resource, please contact Paul Wood at pgw@pitt.edu or (412) 648-9618.

 

For Pitt Biomedical Research Facilities' web site search.

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Drug Discovery Institute (UPDDI)

W950 Biomedical Science Tower
200 Lothrop Street
412-648-9200
https://www.upddi.pitt.edu/

Vision for the University of PIttsburgh Drug Discovery Institute: Optimizing the discovery and development of new molecular entities through collaborations between faculty, academic centers, corporations and government agencies, together with innovations focused on Quantitative Systems Pharmacology (QSP).

The UPDDI has three major classes of activities: a) integrated programs that apply QSP to metastatic breast cancer, Huntington’s Disease (as a first focus area to be extended  to other neurodegenerative diseases) and liver diseases; b) projects based on ligand- or target-centric discovery that are driven by faculty collaborators/ RO1 grants covering many therapeutic areas; and c) translational technology development including companion diagnostics, computational biology/chemistry tools, analytics to identify and quantify heterogeneity in experimental and clinical samples and advanced human tissue models for discovery.  The UPDDI is positioned in an outstanding environment to translate key scientific and clinical advances into novel therapeutics.  

Resources include:

Discovery Technology

Chemistry/Medicinal Chemistry

Computational Biology & Chemistry

Zebrafish Facility

Preclinical Resources

Chemical Biology Facility

Epidemiology Data Center

127 Parran Hall, Epidemiology Data Coordinating Center, Graduate School of Public Health
130 DeSoto Street
412-624-5447
F: 412-624-3775
Website

The Epidemiology Data Center (EDC) provides a research environment in which complex health questions can be explored and answered using the combined tools of biology and statistics. The EDC establishes collaborations with clinical researchers to design, conduct, and analyze multi-center randomized clinical trials and epidemiologic studies. To contribute to the development of new knowledge, the EDC develops and refines data collection, data management, computing, and statistical methods, with the ultimate goal of advancing treatment and prevention of disease.

The EDC has collaborated in over 100 research studies sponsored by the National Institutes of Health and other agencies. At any given time the EDC coordinates data management and analysis activities for 20 to 30 research projects sponsored by federal agencies as well as by industry. The current studies represent a variety of scientific designs including clinical trials, registries, and case control studies.

Genome Editing, Transgenic, and Virus Core (GETV)

Formerly the MWRI Transgenic and Molecular Core (TMC)
204 Craft Avenue; Pittsburgh, PA 15213
(412) 641-2415
Website

The Genome Editing, Transgenic and Virus core (GETV) is dedicated to shepherding projects, from vector design to the establishment and maintenance of valuable transgenic/knockout/knockin lines. The core assists investigators by developing state-of-the-art strategies to solve complex biological problems using transgenic, gene editing and viral vector approaches. Directed by Dr. Kyle Orwig and managed by Dr. Yi Sheng, the core provides technical expertise, advice and services for investigators in the University of Pittsburgh community and beyond. Please see the website or contact us for further information or to schedule a face to face meeting to design and implement your experimental plans.

 Core Services

  • Assistance for generating recombinant plasmids;
  • Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work;
  • Mouse and rat transgenesis, including transgenic and knockout/knockin techniques using pronuclear injection, ES cell injection/chimera and CRISPR/Cas9-mediated genome editing;
  • Lentivirus, adenovirus and adeno-associated virus vectors;
  • Cryopreserve or re-derive of valuable transgenic/knockout or other rodent lines using sperm or embryos;
  • Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines;
  • GETV core services are approved by the Institutional Animal Care and Use Committee (IACUC), the recombinant DNA committee, and the environmental health and safety office of the University of Pittsburgh.

Genomics Research Core

3rd Floor Gold Building
3343 Forbes Avenue
412-648-9940
F: 412-648-1891
Website

The Genomics Research Core is equipped with state-of-the-art instrumentation and provides a variety of standard as well as customized genomic analyses to University researchers. The services include Sanger DNA sequencing, Next Generation sequencing of DNA and RNA, RNA/DNA extraction, purification and QC services, gene expression microarrays, whole transcriptome human and mouse microarrays, miRNA microarrays, TaqMan real-time PCR and Nanostring digital expression profiling for mRNA and miRNA, candidate gene and whole genome SNP array genotyping and NanoString CNV analysis.  New technology and capabilities are added frequently; for a complete description of services and pricing, please visit the website.

The laboratory staff and leadership are available to provide expert advice and support with experimental design, new protocol development, technical support, data analysis and interpretation and assistance with manuscript and grant preparation including budgeting related to Genomics Research Core Services. 

Health Sciences Core Research Facilities

http://www.hscrf.pitt.edu

The Health Sciences Core Research Facilities (HSCRF) were established through the Office of the Senior Vice Chancellor, Health Sciences in recognition of the vital role that centralized research resources play in the academic research enterprise. The technology required to perform innovative biomedical research changes rapidly and centralized research resources provide access to expertise, technology applications, and state-of-the-art instruments that are otherwise unavailable to most investigators.

Our research resource facilities include scientists and technicians who provide expert advice for study design, standard and custom applications and assist the investigator to take their project aims from conception to data production. The scientific leadership is available to work with you to adapt and apply methods appropriate to your scientific goals and assure that the interpretation of the results is supported by the data.

  • Biomedical Mass Spectrometry Center (Proteomics, quantitation & other)
  • Genomics Analysis Core (Bioinformatics)
  • Genomics Research Core (RNA, DNA, Nextgen Sequencing NGS)
  • Health Sciences Metabolomics and Lipidomics Core
  • Health Sciences Sequencing Core at Children's Hospital of Pittsburgh (Illumina sequencing NGS)
  • Pitt Biospecimen Core (formerly Health Sciences Tissue Bank)
  • Peptide and Peptoid Synthesis Core
  • Small Molecule Biomarker Core (Quantitative Mass Spectrometry analysis)

Health Sciences Sequencing Core at Children's Hospital of Pittsburgh

Rangos Research Building 8th Floor
(412) 648-1284 & (412) 692-3555
Website

The Health Sciences Sequencing Core at Children’s Hospital of Pittsburgh (HSSCatCHP) offers Illumina next generation sequencing services to the University of Pittsburgh research community.  The core was established by a cooperative agreement between the University of Pittsburgh Schools of Health Sciences and the Children’s Hospital of Pittsburgh of UPMC.

Our personnel are experts in next generation sequencing technologies and are committed to remaining abreast of advances that will impact the research effort of investigators.  We encourage you to contact our core managers Debby Hollingshead and William Horne to arrange for a consultation.  Our staff is available to share their experience and expertise to assist with development of projects of all sizes, standard or custom assay design, and other specialized application needs.

Luminex Core Laboratory

Lab 1.18 Hillman Cancer Center
5117 Centre Avenue
412-623-7748
F: 412-623-1415
Website

This facility uses the Laboratory Multianalyte Profiling (LabMAP™) system developed by Luminex Corporation. It is a flexible, easy-to-use protein analysis system that permits the simultaneous quantitative analysis of up to 100 different proteins, peptides, or DNA molecules in a single microtiter well. Multiplexing enables a comprehensive overview of complex biological systems and the inclusion of in-process controls using a minimum amount of sample. Potential applications include:

  • functional genomics
  • validation of drug leads
  • secondary screening
  • absorption and distribution
  • metabolism
  • high-throughput ELISA and Western blot assays.

The UPMC Hillman Cancer Center (UHCC) Luminex Core Laboratory can process and analyze samples using commercially available manufactured kits or unique Core developed kits.  The UHCC Luminex Core Laboratory can also develop custom analyte Luminex assays using investigator-supplied antibodies and a protein standard. (formerly UPCI)

Machine Shop

A115 Scaife Hall
412-648-9332
Website

The CBP/Pharmacology Machine Shop provides consultation, design and fabrication services to School of Medicine investigators who need specialized devices for experimental use and specialized clinical devices. The Shop personnel can diagnose and repair mechanical scientific machinery and equipment, and working in conjunction with the CBP/Pharmacology Electronics Shop, can also address the repair of electronically controlled scientific machinery and equipment. Services are fee based and an account is required at the time of request of service.

Magee Womens Research Institute (MWRI) Core Services

For information about each of the MWRIF Core services please go to their web pages or email at the links below.

Flow Cytometry Research Core Web Link

Email: brozickjf@upmc.edu

Genome Editing, Transgenic & Virus (GETV) Core Web Link

Email: korwig@mwri.magee.edu

Histology & Micro-Imaging Core Web Link

Email: ccastro@mwri.magee.edu

Obstetric Specimen Procurement Unit (OSPU)

Email: osbood@mail.magee.edu

Magee Obstetric Medical and Infant (MOMI) Database Web Link

Email: catovjm@mail.magee

Clinical Research and Translational Center (CTRC) Web Link

Email: (klaychak@mail.magee.edu)

Office for Oversight of Anatomic Specimens (OOAS)

Iroquois Building, Suite 300
3600 Forbes Avenue
412-802-8280
http://ooas.pitt.edu/

The Office for Oversight of Anatomic Specimens (OOAS) strives to facilitate and enhance the teaching, training, and research endeavors of our faculty, physicians and staff by creating and fostering an environment based upon the considerate treatment and ethical use of all human cadaveric tissue.

  • The OOAS was established in May, 2007, to provide a centralized support service to faculty, physicians and staff for the approval, procurement, and tracking of human cadaveric tissue required for use in teaching, training and research within the Health Sciences community, as well as for oversight of the utilization, storage, and disposition of tissue.
  • The Committee for Oversight of Research and Clinical Training Involving Decedents (CORID) is administered by the OOAS, and is responsible for the review and approval of all research and educational/clinical training activities that require the use of human cadaveric tissue and decedent medical records.
  • Unless collected through the Pitt Biospecimen Core, all biological materials collected for research purposes, whether utilized for current studies or banked for future research, must be registered with the OOAS.
  • On behalf of the Humanity Gifts Registry of the Commonwealth of Pennsylvania, the OOAS disseminates information to individuals who are interested in body donation.

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